what causes "glowing"?

Ganoderma

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once again, i am having troubles lol.

playing around some more with the sigma 70-300 lens (aka "the cheapy" as my wife calls it).

i am finding myself getting what i want but most of the pictures have this bluing glow type thing to them.

here are some pics i just took now to show. mind you they were hand held at night under medium fluorescent lighting, but the glow happens a fair bit in different situations, even with tripod/remote stillness.

example 1 cactus spines
iso 200
f/5.6
263mm
1/20sec
used flash.

spineglow.jpg




example 2 cactus seeds.
iso 200
f/5.6
263mm
1/20sec
used flash....thinking this has to do with the problem...?
seed1.jpg


cropped
seed2.jpg




another question. i used a white lit box (used for growing seedling plants) and took a pic of various cactus....i used tripod/remot and mirror lockup and took dozens of pics and still not super fine detail.....i am at a loss now :(
i see some excellent pictures of say a bumble bee, and the hairs are in nice detail on said bee....but i cannot (with this one lens) seem to get that kind of detail.

example
iso 100
f/6.3
1/25sec
wool.jpg



any thoughts as to what im doing wrong?
 
are you using external flash or on board pop up flash?
 

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