The subject will be close using extension tubes effectively, but your 50mm example SHOULD focus with the subject 100mm (twice the focal distance) in front of the 1st nodal point. The nodal point will typically be within the lens relatively close behind the front element, it varies on lens design but I'd expect the object distance at 1:1 to be 90-100mm in front of the lenses front surface.
Fit the extension tube & set the lens to manual focus at infinity. Then move the camera slowly closer/further from the subject till the image is in focus. DOF will be very small & the difference the focusing control makes will usually be fairly minimal.
AF can be easily fooled in macro, and you may want to remove the hood to get enough light on the subject...
If you still can't find focus zoom out to 100mm & try again with the extension tube still in place. Focus will be further away& the image less magnified (around 1:2). Having found focus you can zoom in gradually moving the camera closer as you go...
Working distance is often an issue in macro. particularly if shooting bugs. Shooting early in the morning when it's cold helps as the insects need to warm up before they can run away