Focal length comes into play with how close you have to get to the bee to get that 1:1
It might only be a few inches, but with bees, spiders, poisonous snakes, etc... that might be a big deal.
I'm sure it also affects the macro subject similarly as focal length affects non-macro shots. Wider lenses will exagerate distances between near and far objects, and long lenses will flatten the image.