1:1 means that the size of the image on the negative is the same size as the subject, that is, a picture of a bee 1:1 would be life size on the neg, as opposed to 1:3, where it would be 1/3 lifesize.
Focal length comes into play with how close you have to get to the bee to get that 1:1
It might only be a few inches, but with bees, spiders, poisonous snakes, etc... that might be a big deal.
I'm sure it also affects the macro subject similarly as focal length affects non-macro shots. Wider lenses will exagerate distances between near and far objects, and long lenses will flatten the image.