The thing is when you use tubes (as far as I am aware) the actual aperture of the resulting lens is reduced. So whilst you've set the aperture blades to f16, once you add in the tubes you've got an effective aperture that is smaller than f16. This isn't just lightloss, but also an actual aperture change so the depth of field is a little greater and the diffraction effect greater as well. As a result the softness you are seeing might be the result of this change.
However now that I double check your shutter speed and ISO are:
Shot 1 :1/80 ISO 200
Shot 2: 1/100, ISO 400
Now if you've got flash giving you lighting unless you're going to capture natural lighting in the shot as well (for which you need a perfectly still subject and camera - and no wind) you should really put the speed up at the fastest the camera lets you (1/200 or 1/250 at the common values). Also your ISO should be able to go lower, ISO 200 is ok, but 400 will start to show more noise and that might also be partly where you are losing some quality and detail in the second shot.
Again if you're going for more natural light dominance raising the ISO (thus lowering the flash amount needed) helps, at the cost of noise of course.