OK, here’s the drill:
1) pick a well lit spot for your testing, so that low light is not a factor.
2) choose a subject that is contrasty (a lens target, or a newspaper, or anything with fine detail that you can later examine for sharpness).
3) locate that target at about 5 feet away (so that minimum focus distances don’t come into play). Tilt it at a 45 degree angle, and mark clearly the aiming point. This will help you see if the focus if behind or ahead of your intended actual focus point.
4) Place your camera on a tripod. If necessary, weight down the tripod to make sure it doesn’t budge.
5) Put your 24-105 lens and turn AF off and IS off. You don’t want to rely on the camera’s autofocus ability, and you don’t want the possible jiggling that IS can make while it’s searching for stability.
6) Take off any filter you may have on. Some will cause issues with the picture.
7) Focus on your chosen target using live-view and the 10x magnification. Make sure that you’ve clearly focused on the chosen spot.
8) Choose the aperture of f/4. Any focusing issues will be more obvious at minimum DOF.
9) Set your shooting mode to manual, and set your shutter based on whatever meter reading your meter gives you.
10) Lock up your mirror (see subscuck’s suggestion).
11) Trigger your camera with the remote.
12) Examine the resulting photo & histogram, making sure that the key focus area is well exposed. If not, adjust exposure and re-shoot.
13) If you have another camera lens, put it on at this point and go through steps 6 though 12. The focal length should be close to what you used on the 24-105mm lens (so if you shot that lens at 50mm, the comparision lens should also be at 50mm). F/stop should also be the same, if at all possible.
14) Bring in the RAW files into your computer and process them exactly the same way, ie, use the exact same amount of sharpening, etc. with all (so that post-processing is a non-factor).
15) Now compare. Look specifically at your focus point, and the area around it and do a side-by-side comparison at maybe 400% to see how the detail shows up. If you want our feedback, crop out the center 600x600 pixel squares at 100%, and post these.