Well, usually I use my flash on an off-camera cord with a Lumiquest softbox (Camera is a 7D and the lens is a Canon 100 mm f/2.8 Macro). In this case, however, I just handheld the camera in natural light. f/11 @ 1/250 sec ISO 250. I wasn't really thinking, or I would have upped the ISO and shutter speed, but I got lucky. No set-up. I didn't really appreciate those cool eyes until I saw it on the screen.