Quote:
The D300 allows user controllable ISO values from 200 to 3200, in as little as one-third stop steps (you can alter the settings to half or full stops using Custom Setting #B1 [see page <561>], but I'd suggest just leaving the camera at the default).
The D300 also has settings of
LO 0.3, LO 0.7, and LO 1.0, HI 0.3, HI 0.7 and
HI 1.0, which are approximately equivalent to ISO 100, 125, 160, 4000, 5000 and 6400 respectively. These last six values are not labeled with an ISO value because they have image quality limitations that the numbered ISO values do not. The non-numbered ISO values are a warning to you that some aspect of image quality will be compromised:
- LO values: the LO ISO values compromise highlights. What tends to happen is that highlight data gets "clipped" in high contrast scenes: (chart illustrating the effect)
Think of it like this: the image is overexposed at ISO100 by a stop, then data pulled back downwards to adjust for that. This has two primary effects: first, bright highlights that were near blowing out at ISO200 are now blown out; and second, noise is somewhat buried in the deep shadows. In my experience, LO values work okay for low contrast scenes, but are dangerous for high contrast scenes due to the loss of highlight information. Moreover, if you look closely on long exposures, you often see artifacts at LO values that you don't at ISO200.
- HI values: the HI ISO values compromise overall camera dynamic range. As you move ISO values up, the camera dynamic range decreases. Eventually, that range becomes suspect and it gets difficult to reproduce a wide range of tones. Nikon marks that changeover point by stopping the use of numeric ISO values: (chart illustrating the effect)