What settings are you shooting at? If you are shooting at a slow shutter speed, that could be your problem. Also, if you were on a tripod for the first shot, was the stabilization turned off on your lens? ( it should be when attached to a tripod ) Also, read your manual and learn how to lock the mirror ( or use live view ) This will eliminate the possibility that the slap of the mirror is causing the camera to vibrate. You were at 1/13th of a second so this is a possibility.
Also, as you get high on the F/stops there will be a point with which your lens starts to degrade due to defraction. Not sure about your specific lens, but I would wager that sharpness is probably starting to decline at f/16.