Kinda wondering what you're trying to figure out. Is it the AF accuracy, the lens focusing ability, or something else?
When doing tests, you try to keep all variables steady, and change only one to see what the effect is. In this case, putting the camera on a tripod is essential, both to eliminate any camera shake as a reason for blur, and secondly to allow you to use manual focus. Turn your AF off, and use live-view (10x) to focus on the key point (say the 3.5" mark). Use either a remote release, or 2-second shutter release delay to trigger the shutter. By eliminating possible sources of camera movement, you make your focus test more reliable. Of course, the more light you have, the easier it is to focus and also to get a good exposure. Once you've achieved the best focus possible with the live-view focusing, you can switch to your AF, enabling ONLY the center point, and using that on your previous target to see if the camera/lens combination achieves as good a focus as you were able to achieve manually. If yes, excellent. If no, then you start checking if you have front/back-focusing issues.