Highest ISO you feel comfortable with. Longer prime lenses of f/2.8 would be idea, using those fast lenses from farther away, like 135mm from 15-30 rows back, or the wings, 200mm from the balcony. Keep in mind that under flood and spot lighting with a dark background, actors in the spot/flood/bright light will be terrible OVER-exposed if the camera's light meter is allowed to "see" much of the darker, not-lighted area of the stage or curtains, back set etc.etc. I see you have the normal 2-kit zoom setup, so crank the ISO to 1600 or so most likely.
Your IS lens could be used to get some neat blurred effects, or some panning. The thing is, your lenses lose aperture as they are zoomed, so I would suggest shooting them at the shorter settings, where they admit more light. Use Aperture Priority, not shutter, if you use auto.
This means that in many situations, Automatic exposure will need to have Minus Exposure Compensation dialed in, if the meter is reading large expanses of dark curtains, flood- or spot-lighted performers can easily require a Minus 3.0 stop +/- favtor dialled in to correct for the meter's tendency to over-expose. I cannot imagine having to shoot much of this kind of stuff with a lens that only lets in f/5.6...there will most likely be significant quantities of ruined shots and almost-but-not-quite-sharp-enough type pics, due to subject movement blur.