I mentioned using a light meter on your phone to test lens aperture & ISO settings in your situation.
I'm a newbie at light meters but it can really help in understanding ISO, Aperture and shutter speeds without having to take tons of photos and comparing them.
So to very poorly emulate your situation, I selected this fast moving monkey and giraffe

jumping up and down on a stool in my basement.
Here's an example in Low light and using a iPhone light meter
In a low light situation, one flourescent behind me in my basement - quite dim
On the first one. I check the lighting with ISO 1600 and aperture 5.6. The light meter tells me the proper exposure would be shutter at 1/8
2nd - ISO 1600, f/2.8 then shutter at 1/30


So the larger aperture opening of f/2.8 allows me to go from 1/8 shutter to 1/30.
If I add a little light to the subject - which is still low light but MORE than before
ISO 1600, F/2.8 the shutter speed increases to 1/125
Each light meter results gives me the manual settings for the camera, and the exposure is fine without having to hunt and peck settings.
I could also bump up the ISO, which will allow me even faster shutter speed.
So I now push the ISO to 4000, f/2.8 and the resulting shutter speed is 1/250
But this example shows you low light and different aperture settings.
It can also show you the limitations of your current equipment based on the lens itself.
Of course, the cameras ISO capabilities also come into play.